virulence function of pectobacterium atrosepticum secretion system mutants on evaluation of some solanum tuberosum resistance genes
نویسندگان
چکیده
background: p. atrosepticum is a commercially important pathogen. it causes blackleg in the field and soft rot of tubers after the harvest. this effect is due to secretion of depolymerases and other virulence factors by several mechanisms including t3ss objectives: the effect of bacterial t3ss on solanum tuberosum (s. tuberosum) varieties and its relationship with s. tuberosum resistance gene expression were studied. materials and methods: a p. atrosepticum hrpw gene was cloned, sequenced and constructed a phylogenic tree with some phytopathogen. the virulence properties of p. atrosepticum strains were investigated and then s. tuberosum varieties were tested for their sensitivity against p. atrosepticum. pr-5 and hin genes copy-number for infiltrated s. tuberosum tubers were assessed. results: the results show that infiltrated tubers of p. atrosepticum t3ss mutants were significantly more macerated than the wild type ones. conclusions: pr-5 and hin expression amounts were depended on bacterial t3ss function.
منابع مشابه
Virulence Function of Pectobacterium atrosepticum Secretion System Mutants on Evaluation of Some Solanum tuberosum Resistance Genes
Background: P. atrosepticum is a commercially important pathogen. It causes blackleg in the field and soft rot of tubers after the harvest. This effect is due to secretion of depolymerases and other virulence factors by several mechanisms including T3SS Objectives: The effect of bacterial T3SS on Solanum tuberosum (S. tuberosum) varieties and its re...
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متن کاملGenome Sequence of the Pectobacterium atrosepticum Strain CFBP6276, Causing Blackleg and Soft Rot Diseases on Potato Plants and Tubers
Pectobacterium atrosepticum strain CFBP6276 is a pectinolytic enterobacterium causing blackleg and soft rot of the stem and tuber of Solanum tuberosum. Its virulence is under the control of quorum sensing, with N-acylhomoserine lactones as communication signals. Here, we report the genome sequence of P. atrosepticum strain CFBP6276.
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عنوان ژورنال:
iranian journal of biotechnologyناشر: national institute of genetic engineering and biotechnology
ISSN 1728-3043
دوره 11
شماره 2 2013
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